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You won't find an undo command to return your document to a single-column format in Word 2010.
But you can undo multiple columns in a document by clicking the Page Layout tab, clicking Columns, and then clicking One to reformat your entire document as one column.
Do you have Word tips or tricks to share? Send them our way.
--Leslie H. Cole
Don't forget about the side effect of such technique: part of document that you return to one column layout will be still enclosed in section breaks. That can hit back if you tend to maintain secton-dependent headers and footers.
Thanks for the reminder, drgrba!